A Label-Free Molecularly Imprinted Electrochemical Sensor Based on MXene Nanosheets Modified by Gold Nanoparticles for Sensitive and Selective Detection of Homocysteine

A label-free molecularly imprinted electrochemical sensor (MIECS) based on electropolymerized polymer (MIP) was developed for the determination of homocysteine (Hcy) in serum first time. MXene@AuNPs with layered structure synthesized electrode by modifying MXene gold nanoparticles (AuNPs). MIP dopamine hydrochloride (DA) were onto surface modified molecular docking and quantum chemical calculations specific recognition Hcy. as a carrier immobilizing steadily enhances conductivity (about 4.2-fold) plays crucial part improving detection sensitivity MIECS. The results showed that current response linearly decreased increasing concentration Hcy range from 1 × 10 −13 to −5 mol l −1 , limit (LOD) quantification (LOQ) 11.81 fmol 39.49 respectively. With favorable selectivity, stability, reproducibility ruggedness, MIECS applied human samples recoveries 87.83%–92.58%. proposed strategy has potential application disease surveillance.